Chromatographie Studies of Tyrosyl and Phenylalanyl Transfer RNA's of Liver and Tumor Cells1
نویسندگان
چکیده
from several normal and cancerous tissues were studied by means of the newly developed reverse-phase chromatography system, RP-5. Tyrosyl transfer RNA from normal rat, mouse, and hamster livers emerged as a single peak. All three species exhibited identical profiles. Tyrosyl transfer RNA from Escherichia coli required a considerably higher NaCl concentra tion for elution from the RP-5 column. Tyrosyl transfer RNA from the Novikoff ascites hepatoma emerged as two separate peaks—one identical with the normal liver and a second late-eluting peak. Tyrosyl transfer RNA from SV40-induced tumors in hamsters and also from Ehrlich ascites tumor cells exhibited a broad-based peak which eluted later than the normal and which probably consists of two or more tyrosyl transfer RNA's.
منابع مشابه
Transfer RNA species in tumors of different growth rates.
Tyrosyl-, histidyl-, lysyl-, and phenylalanyl-tRNA's from 3 tumors (DBAH, DBAH, and DBA), differing in growth rates and from host mammary glands and liver, were compared by means of methylated albumin kieselguhr (MAK) column and by reverse-phase-5 chromatography. The elution profiles of lysyl-tRNA's from DBAH and DBA, phenylalanyl Trna's from DBAH and DBAH, and histidyl-tRNA's from DBA3 tumors ...
متن کاملIsoaccepting transfer RNA's in mammalian differentiated cells and tumor tissues.
amino acids derived from the MOPC 31C plasma cell tumor and resolved by the RPC-2 column chromatography was published (28). Except for tryptophan, multiple peaks of isoaccepting tRNA were observed for each of the other 19 amino acids, each having its characteristic elution pattern. As compared to E. coli studied under similar Chromatographie conditions (24), MOPC 31C plasma cell tumors showed v...
متن کاملPattern of isoaccepting transfer RNAs common to 26 patients with Hodgkin's disease.
The elution profiles of aminoacyl transfer RNAs from Hodgkin's tumors have been compared with the corresponding patterns from normal splenic tissue. Aminohexyl-Sepharose and reversed-phase 5 chromatography have been used in the fractionation studies. Three peaks of acceptor activity have been observed in phenylalanyl, histidyl, aspartyl, and asparaginyl transfer RNAs. A second peak was shown in...
متن کاملThe fractionation of transfer ribonucleic Acid from roots of pea seedlings.
Isoaccepting transfer RNA species for several amino acids were fractionated by reverse phase column chromatography. Transfer RNA from dividing cells of pea (Pisum sativum) root was compared to that from nondividing cells, and no relative quantitative or qualitative differences were noted for the transfer RNA species for leucine, lysine, proline, threonine, methionine, serine, and phenylalanine....
متن کامل